![]() ![]() Nevertheless, biological tissues are composed of wide variety, heterogeneous and anisotropic structures and, consequently, predicting the applied electric field distribution is complex. It is necessary to apply an optimized transmembrane potential at each point of the tissue by means of a homogenous electric field application and appropriated electric field orientation. ![]() In this area, it is essential to apply homogeneous treatments to achieve complete removal of tumors and avoid relapse. Nowadays, its clinical application to cancer treatment is one of the most relevant branches within the many areas of electroporation. The system offers high flexibility in experimental design to trigger various electroporation-based phenomena.Įlectroporation is a phenomenon that consists of increasing the permeability of the cell membrane by means of high-intensity electric field application. Based on a YO-PRO-1 permeabilization assay, it was determined that the electroporator is suitable for applied research on electroporation. The electroporator was experimentally tested on the H69AR human lung cancer cell line using 20 kV/cm bipolar and unipolar 100 ns-1 μs pulses. The shortest pulse duration step is hardware limited to 33 ns. The resultant circuit generates pulses with repetition frequencies up to 5 MHz and supports unipolar, bipolar, and asymmetrical pulse sequences with arbitrary waveforms. A synchronized double crowbar driving sequence is introduced to generate short nanosecond range pulses independently of the electroporator load. The electroporator supports voltages up to 3 kV and currents up to 40 A and is based on H-bridge circuit topology. In this work, a novel electroporation system (electroporator) is presented, which is capable of forming high frequency pulses in a broad range of parameters (65 ns-100 μs). ![]()
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